Following a Bank Transfer (ACH) deposit (USD only), the deposit limit will be reset after 24 hours. ACH deposit requests are submitted at 5pm ET Monday - Friday at 24-hour intervals.
Any deposit made before 5pm ET will be submitted to your bank that same day, and any deposit made after 5pm ET will be submitted to your bank at 5pm ET the following business day.

Note: Deposit limits are not reset on the weekend or on Federal U.S. holidays.